Enzyme Activity

LABORATORY REPORT (Click on the Save a write expiration on the panel above to save your report) activeness: give: Instructor: Date: Enzyme Activity Khadija Mohamed Dr Smith celestial latitude 10, 2011 Predictions 1. invertase will clear the greatest legal act at pH 6 2. invertase will have the greatest action at 40 °C (104 °F) 3. Sucrase activity change magnitudes with change magnitude saccharose concentration until a plateau is reached. Materials and Methods put in of pH on Enzyme Activity. 1. interdependent Variable. pith of product (glucose and fructose) produced 2. breakaway Variable. pH 3. Controlled Variables. temperature; amount of substrate ( sucrose) arrive at over; invertase + sucrose pensiveness time Effect of Temperature on Enzyme Activity. 1. dependant Variable. amount of product (glucose and fructose) produced 2. Independent Variable. temperature 3. Controlled Variables. pH; amount of substrate (sucrose) lay; saccharase + sucrose incubation time Effect of Substrate Concentration on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. amount of substrate (sucrose) present 3. Controlled Variables. temperature; pH; saccharase + sucrose incubation time 1.

let on what is measured as an index of sucrase activity and why this is an indicator of sucrase activity. Glucose is measured as an indicator of sucrase as glucose is a product produced by sucrase activity. 2. explicate why denatured sucrase was used as a control. to determine if a dependable effect was make on the active sucrase by ph , temperature and sucrose or if fall would ! be seen on both denaturated and active sucrase 3. Alkaline DNS denatures sucrase. apologize why it is burning(prenominal) to denature the enzyme. It is important to determine if ph, temperature and sucrose do have a true effect on active sucrase. leaving it active would increase the activity of the enzyme Results submit across 1: Effect of pH on Sucrase Activity 1 2 3 average ph 3 0.1816 0.1873 0.1538 0.174 ph 4...If you want to get a full essay, order it on our website: OrderCustomPaper.com

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